A PROTOCOL FOR THE DETECTION OF GENETIC MARKERS IN SALIVA BY POLYMERASE CHAIN REACTION WITHOUT A NUCLEIC ACID PURIFICATION STEP: EXAMPLES OF SARS-COV-2 AND GAPDH MARKERS

Introduction. Polymerase chain reaction (PCR)-based diagnostic tests use purifi ed nucleic acids (NAs) from clinical samples. The NAs cation step adds time, cost, and aff ects the quality of testing. objective this study was to develop a protocol for direct saliva in genetic markers, without acids. Methods. PCR, real-time RT-PCR isothermal amplifi were used detection Results. We report markers saliva. is based on collection solution containing detergent ethanol compatible with (LAMP), RT-PCR. SARS-CoV-2 GAPDH as reference markers. observed that mild detergents allow effi cient external intracellular endogenous while strong detergent, e.g. sodium dodecyl sulfate, inhibited PCR reaction. Under these conditions, samples can be stored 24 h at +4°C or –18°C preservation Storage room temperature led deterioration marker detection. Snap heating time collection, followed by storage temperature, provided partial protection. Conclusion. presented describes LAMP tests.